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Authors
Affiliations
1 Department of Quality Assurance, P.W. College of Pharmacy, Dhamangaon Road, Yavatmal, 445001, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 12 (2011), Pagination: 1829-1832
Abstract
A simple isocratic RP-HPLC method has been developed and subsequently validated for the determination of Ramipril and Amlodipine Besylate in pharmaceutical dosage forms within very short retention time. The method employs an Xterra C18 column, 5 μ, 150 mm x 4.60 mm id with flow rate of 1.5 ml/min using UV detection at 210nm. The separation was carried out using a mobile phase consisting of Sodium Lauryl Sulfate buffer by adjusting pH 2.5 and final composition is Buffer: Acetonitrile: Methanol (45:16.5:38.5)V/V. The retention time for Ramipril and Amlodipine Besylate was found to be 3.1 minutes and 3.8 minutes respectively. The results of analysis were validated statically and by recovery studies. Hence the proposed method was found to be accurate, precise, reproducible and specific and can be used for simultaneous analysis of these drugs in tablet formulation.
Keywords
RP-HPLC, Sodium Lauryl Sulfate Buffer, Ramipril, Amlodipine Besylate.
